An active-site titrant for human tissue-type plasminogen activator.
نویسنده
چکیده
The reaction of recombinant tissue-type plasminogen activator with the inverse substrate 4-amidino-2-nitrophenyl 4'-anisate results in the rapid release of the chromogen 4-amidino-2-nitrophenol and the accumulation of the relatively stable 4-anisoyl-enzyme. Spectrophotometric monitoring of the reaction enables the operational molarity of the enzyme to be determined.
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عنوان ژورنال:
- The Biochemical journal
دوره 239 2 شماره
صفحات -
تاریخ انتشار 1986